3D relief imaging

Comparison of different observing methods

The gallery contains a comparison of sample images obtained by different observation methods - in a bright field, with relief contrast with a help of LUMICRA sliders (Classic or Tunable) and with phase contrast (standard Leica phase slider). Various preprepared sample slides (without staining or stained) were used for the imaging. These slides are intended mainly for observations with phase contrast, or by DIC and polarizing microscopy. Images were taken with a DM750 microscope equipped with Leica IIC50 or FLEXACAM C1 cameras. The resolution for the display has been reduced. Original images can be supplied on request.

A significant relief effect is evident in images taken with LUMICRA sliders. This contrast methond allows much easier observations of the tissue, cells or organisms with hightlighted details on the surface.

To compare images drag the blue element with the arrows to the left and right.

Diatoms - Relief Contrast vs. bright-field

Increase of contrast and details with LUMICRA sliders, 20X magnification

Brightfield Brightfield
Relief Contrast Relief Contrast

Diatoms - Relief Contrast vs. Phase Contrast

comparison of different contrast methods, 20X magnification

Phase contrast Phase Contrast
Relief Contrast Relief Contrast

Advantageous increase in contrast with LUMICRA Relief Contrast

Tissue slices (domestic cat, tongule: longitudinal), 20X magnification

Brightfield Brightfield
Relief Contrast Relief Contrast

Different contrast methods - Relief Contrast vs. phase contrast

Tissue slices (domestic cat, tongule: longitudinal), 20X magnification

hase contrast Phase Contrast
Relief Contrast Relief Contrast

Relief contrast achieved by advanced oblique illumination

Tissue slices (domestic cat, tongule: longitudinal), 20X magnification, small aperture with brightfield

Brightfield Brightfield, small aperture
Relief Contrast Relief Contrast

Relief Contrast - contrast enhancement with LUMICRA sliders

Tissue slices (domestic cat, tongule: longitudinal), 40X magnification, large aperture with brightfield

Brightfield Brightfield, large aperture
Relief Contrast - strong Relief Contrast

comparison of oblique illumination with phase contrast

Tissue slices (domestic cat, tongule: longitudinal), 40X magnification

Phase contrast Phase Contrast
Relief Contrast Relief Contrast

See all the images in our classic image gallery. Continue here.